Perfect Tools for Pristine Oligos

Custom Radiolabeling of Synthetic Oligonucleotides

AM Chemicals offers services in expert synthesis of                        radiolabeled oligonucleotides and their phosphorothioate analogs      (1 to 100 µmol of isolated product) including:

  • Synthesis of                     -labeled, protected nucleoside phosphoramidite building blocks

  • Synthesis of the target radiolabeled oligonucleotides

  • Purification by reverse-phase or ion-exchange HPLC as requested by customer and desalting

  • For siRNA, forming a duplex by hybridization with the unlabeled strand

  • Optionally, confirmation of structure by ES MS

  • Optionally, removal of endotoxin from the final product

  • Optionally, performing above operations under GMP conditions

 

Custom Quote

AM Chemicals is pleased to provide a custom quote for radiolabeled oligonucleotide synthesis project.

To request the custom quote, please furnish the following information:

  • Oligonucleotide sequence

  • Desired radioisotope (                        )

  • Placement of the radiolabeled nucleoside in the sequence

  • Desired specific activity (SA):

    • A single labeling affords SA of 30-40, 100-400, and 500-1500 Ci/mol for                       radioisotopes, respectively

  • Desired amount of radiolabel incorporated or amount of radiolabeled oligonucleotide

  • Customers’ ability to provide project-specific unlabeled phosphoramidites of modified nucleosides or non-nucleosidic moieties

Guidelines for Optimal Cost Control of Radio-labeling Project

  • Awareness of radiolytic degradation saves money: aim for the specific activity of the target oligonucleotide as low as permissible

  • Use           -thymidine or      as carriers of the radiolabel

  • Placement of the radiolabeled moiety: close to the 5'-terminus of the target oligonucleotide

  • Provide project-specific unlabeled phosphoramidites of nucleosides or non-nucleosidic moieties

  • For shipping, solution is a preferred physical state of the finished product (oligo)

  • Acknowledge lead time for radio-labeling project (8 to 12 weeks) and plan ahead