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Custom Radiolabeling of Synthetic Oligonucleotides

AM Chemicals offers services in expert synthesis of                        radiolabeled oligonucleotides and their phosphorothioate analogs      (1 to 100 µmol of isolated product) including:

  • Synthesis of                     -labeled, protected nucleoside phosphoramidite building blocks

  • Synthesis of the target radiolabeled oligonucleotides

  • Purification by reverse-phase or ion-exchange HPLC as requested by customer and desalting

  • For siRNA, forming a duplex by hybridization with the unlabeled strand

  • Optionally, confirmation of structure by ES MS

  • Optionally, removal of endotoxin from the final product

  • Optionally, performing above operations under GMP conditions


Custom Quote

AM Chemicals is pleased to provide a custom quote for radiolabeled oligonucleotide synthesis project.

To request the custom quote, please furnish the following information:

  • Oligonucleotide sequence

  • Desired radioisotope (                        )

  • Placement of the radiolabeled nucleoside in the sequence

  • Desired specific activity (SA):

    • A single labeling affords SA of 30-40, 100-400, and 500-1500 Ci/mol for                       radioisotopes, respectively

  • Desired amount of radiolabel incorporated or amount of radiolabeled oligonucleotide

  • Customers’ ability to provide project-specific unlabeled phosphoramidites of modified nucleosides or non-nucleosidic moieties

Guidelines for Optimal Cost Control of Radio-labeling Project

  • Awareness of radiolytic degradation saves money: aim for the specific activity of the target oligonucleotide as low as permissible

  • Use           -thymidine or      as carriers of the radiolabel

  • Placement of the radiolabeled moiety: close to the 5'-terminus of the target oligonucleotide

  • Provide project-specific unlabeled phosphoramidites of nucleosides or non-nucleosidic moieties

  • For shipping, solution is a preferred physical state of the finished product (oligo)

  • Acknowledge lead time for radio-labeling project (8 to 12 weeks) and plan ahead

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